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DECAPSULATION

Definition

A process whereby the capsules of brine shrimp cysts are removed before they are used further in cultivation. The cyst, often called an egg, is an arrested gastrula encapsulated within a hard lipoproteinaceous shell or capsule. An efficient method of hatching Artemia is to decapsulate the cysts with the hypochlorite solution (OCl-, supplied by chlorine bleach) prior to incubation and hatching. Hypochlorite chemically removes the capsule without affecting the viability of the unhatched embryo. Decapsulation has the added advantage of disinfecting the cysts prior to hatching, eliminating the risk of introducing exogenous pathogenic organisms that may adhere to the outer cyst layer.

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